The Obvious Advantages of Contracting Out Your IHC Histopathology

Overview

Histology and Histopathology Research is the study of the microscopic anatomy of cells and tissues of plants and animals. It involves the use of a microscope to study the structure and function of cells, tissues, and organs. Histopathology techniques are used to diagnose disease and study the effects of therapeutic interventions. Histopathology involves the study of the microscopic features of diseased tissue and the interpretation of these features to make a diagnosis. IHC (Immunohistochemistry) is a challenging procedure that necessitates advance planning to ensure that tissues are correctly gathered and preserved before you begin. Your IHC might be assisted by a reliable outsourcing partner who will provide reliable, reproducible outcomes.

Each stage of your IHC study, from tissue collection to data analysis, will have an effect on how it turns out. You can get assistance from our highly skilled and qualified staff of pathologists, scientists, and histologists at every stage.

The obvious advantages of contracting out your IHC histopathology work include cost savings, increased efficiency, and improved accuracy. Contracting out IHC histopathology work allows laboratories to reduce costs by eliminating the need to purchase and maintain expensive equipment and materials. It also allows laboratories to benefit from the expertise and experience of experienced IHC histopathologists. Contracting out IHC histopathology work can also help to increase efficiency and accuracy, as experienced IHC histopathologists are able to analyze a high volume of slides quickly and accurately.

Tissue Collection and Fixation is your Primary Concern

Your tissue fixation requirements may change based on the marker you choose to examine. Whereas most antibodies only function on frozen tissues, some are effective on formalin-fixed paraffin-embedded (FFPE) tissues as well. You need to process and embed your samples after you've fixed them. In order to preserve important materials, embedding in paraffin can be messy and requires experience with the process. Damaged portions will result from an insufficient paraffin infiltration. It can also be challenging to embed in OCT for frozen parts. To make sure the OCT is correctly frozen and that the media is free of air bubbles, check both of these things. After your samples have been appropriately embedded, you should have a skilled histologist cut your sections. Obtaining tissue sections free of creases, rips, folds, and other artefacts is extremely difficult and demands a skilled touch. Tissue spacing on a slide is crucial for good staining and to avoid cross-contamination.

The Selection of Antibodies is Crucial

There are several commercially available antibodies for the majority of proteins. How do you decide which to pick? Experience and a basic knowledge of IHC concepts are necessary for this. Do the antibody's reactions to the particular epitope in question? are just a few of the topics we could ponder. Has the antibody been examined in the research species? Is the antibody monoclonal or polyclonal? What species of host does the antibody have? Which technique for antigen retrieval is most suitable for my antibody and tissue? How much of my antibody will I need to use at what concentration? Which controls are appropriate for my IHC? Is multiplexing necessary?

These are only a handful of the numerous queries and information that may be used to optimise an IHC procedure. You have to believe in repeatable procedures carried out by professionals employing an automated staining system for this reason.

Processing Samples Consistently

What if you're trying to figure out whether two proteins are co-localized in a cell? Two antibodies must be carefully optimized on the same tissue under comparable circumstances. This procedure can use up a lot of antibodies and reagents if it isn't done correctly. Key is consistency. With the price of antibodies and other reagents, testing dilutions and establishing methods won't be something you want to be doing when you have crucial samples to process merely waiting in the pipeline. Our team has a lot of expertise with dual immunofluorescent labelling and does it frequently.

By giving us the time to process your samples, you may focus on other parts of your business while saving time. We can do your task quickly and effectively thanks to our expert staff, which will save you both time and money.

We have skilled pathologists and scientists on hand to talk about your research requirements and outcomes and assist you in choosing the appropriate IHC markers to employ to accomplish your objectives. We have several procedures in place and have a lot of experience creating new ones to match your needs.

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