HPLC is Useful Instrument

Overview

The Analytical Chemistry: An Indian Journal is a peer-reviewed, open-access academic journal that provides worldwide distribution of original, distinctive scientific publications based on the many fields of agriculture and medicine to discuss a variety of brand-new challenges in analytical chemistry.

The Analytical Chemistry: An Indian Journal is dedicated to the prompt publication of significant original research in the basic theory, practice, and application of analytical and bioanalytical science, including miniaturization of analytical systems, bioanalyses, chromatography, mass spectrometry, electrophoresis, electrochemistry, sampling, and sample handling, and atomic and molecular spectroscopy. All submissions will go through a thorough peer review process, with the calibre, creativity, and range of reader interest being taken into account. The publication upholds its scientific value by disseminating the most significant recent findings in all fields of analytical chemistry.

Recently In our upcoming issue, we published an article entitled “A SIMPLE HPLC METHOD FOR THE ESTIMATION OF ANDROGRAPHOLIDE’’ by one of the eminent authors “Yedugani Lingam” Bhoomi Nutraceuticals Pvt Ltd, Research and Development Centre.  

This article is about “ESTIMATION OF ANDROGRAPHOLIDE” by HPLC method in which we used the mobile phase Water: Acetonitrile (60:40) Flow rate 1.0 ml/min. The retention time of pure Andrographolide was 5.038 minutes. Since ancient times, medicinal plants with a natural and traditional history have been utilized to cure a wide range of illnesses and ailments. The herb Andrographis paniculata contains an active ingredient called andrographolide, which can have biological effects. It is used in several ayurvedic dose formulations. These plants are used to make a variety of herbal compositions to treat a variety of illnesses since they contain several active components.

The elements of the andrographilide's basic materials have a wide range of pharmacological effects. Chemically, andrographolide has a lactone ring made of a bicyclic diterpenoid. Its primary uses include antibacterial, anti-inflammatory, hepatoprotective, antioxidant, and anti-fungal properties. It is employed in the treatment of many ailments and is available in the form of numerous ayurvedic formulations due to its vast range of biological activity. So in the Ayurveda businesses, quality control of formulations containing andrographolide is crucial. From dried Andrographis paniculata, andrographolide was extracted. The local market provided the plant's raw material (BNPL/RMAD/001), which has a moisture level of 11.2%. Water, Acetonitrile, and Methanol

Because methanol is a freely soluble source of andrographolide, methanol was used to extract the plant material (BNPL/RMAD/001). HPLC's mobile phase was Water: Acetonitrile (60: 40). The initial run was blank to check for mobile phase errors.

The reasonably pure andrographilide material (BNPL/AD/006) is eluting at a retention time of 5.038 under the current HPLC conditions, and some nonpolar impurities were obtained at retention times of 6.413, 6.642, and 9.278 accordingly.

By using the Karl-Fischer method, it was discovered that the isolated medication had a moisture content of 0.34%.

In the current study, an HPLC method for andrographolide determination in both raw and pure materials was devised. Methanol was utilized to extract andrographolide, and a Phenomenex C185u, (4.6X250 mm) column was employed. The sample was measured at 223 nm using water: acetonitrile mobile phase (60:40). This is a small variation of the procedure outlined by earlier authors who utilized detection at 254 nm and a mobile phase of chloroform: methanol (9:1 v/v). Due to the compound's high peak and improved resolution at 223 nm, this change was made. The study's findings intended to ascertain the amount of andrographolide in both raw and pure material.

The devised HPLC method is particularly sensitive, dependable, and accurate for estimating andrographolide in diverse crude materials. For the accurate assessment of the many pharmacokinetic characteristics of this beneficial phytochemical, a sensitive approach like this one is required. This approach will be very helpful for planning the next human clinical trials for andrographolide.

The newly created HPLC analytical technique is straightforward, focused, and selective for figuring out andrographolide in bulk. For frequent usage in the herbal drug sector, it was discovered that the developed method is accurate, has greater resolution, has a strong peak, is robust, stable, and is cost-effective.

Fast Editorial Execution and Review Process

Analytical Chemistry: An Indian Journal is participating in the Fast Editorial Execution and Review Process (FEE-Review Process) with an additional prepayment of $99 on top of the usual article processing fee. Fast Editorial Execution and Review Process is a special service that enables the handling editor to respond to the article more quickly during the pre-review phase and to provide a review. After submission, pre-reviews can be finished in as little as 3 days, while reviewer reviews can take up to 5 days. After that, publication and revisions can be finished in as little as two days. Once the handling editor or the previous reviewer is informed that the article has to be changed, it will take them an extra 5 days to assess it.

We also provide the membership plan, if you have more than 2-3 articles. 

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